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Changes in gene expression and protein abundance between the parent wild-type strain and each isogenic mutant were evaluated across the four growth conditions.
Next, transcript levels of the psbK and ndhF genes in the Ossig1 mutant were evaluated by qRT-PCR and found to be 30% and 21% lower than WT, respectively (fig. 6 D).
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The secondary structure of the cTnC mutant was evaluated by circular dichroism, which did not indicate global changes in structure.
In the second screening, M3 generation of each candidate mutant was evaluated for their resistance to Al based on relative root elongation (RRE).
Therefore, the hfq deletion mutant was evaluated for its efficacy as a potential vaccine strain.
The response of primary human melanocytes to the oncogenic, melanoma-associated N-RASQ61K mutant was evaluated by stably transducing N-RASQ61K into human epidermal melanocytes.
MK-0457, a pan-aurora kinase inhibitor that also inhibits the ABL T315I mutant, was evaluated to treat patients with chronic myelogenous leukemia (CML) or Philadelphia chromosome (Ph+) acute lymphoblastic leukemia (ALL) with the T315I mutation.
Next, the generalizability of the 202 08 intein mutant was evaluated by testing effects on display and activity after its fusion to GFP and a cohort of 7 additional scFvs.
We believe that intrinsic limits on precision of Autodock scoring would not be a major concern as both the wild type and the mutant are evaluated using the same scoring scheme and the cut-off has been chosen on basis of native protein-ligand complexes in CSAR and PDBbind datasets.
With this assay, 40 human MAT mutants were evaluated against 7 SAAMs as potential substrates.
The binding affinities of WT and Lon mutants were evaluated by determining the concentration of λ N (KλN) needed to achieve 50% maximal ATPase stimulation.
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