The product tolerance of the NHaseM (αS122C) mutant was enhanced while its activity decreased by 30%.
The 2,3-butanediol production from sugarcane molasses by the mutant was enhanced by 60% in batch fermentation compared to that by the wild type strain.
The specific activities of both double-mutants (M222A/I31L and T220S/I31L) were significantly increased when compared with the single-mutants (M222A and T220S) and the oxidative stability of M222A/I31L mutant was enhanced with respect to wild-type NK.
Bisht et al. (2013) also reported that the activity of alkaline lipase from Pseudomonas aeruginosa mutant was enhanced in the presence of hydrogen peroxide with residual activity of 104.5%.
The alka e)ne production of LX57 mutant was enhanced by 3.6 times when compared to Synechocystis sp. PCC6803.
Upon CUL1-neddylation, the interaction with wild-type UBXN7 and also with the ΔUBA mutant was enhanced, while the interaction with the ΔUIM mutant remained unaffected.
Interestingly, the fluorescence of the labelled V216C mutant is enhanced instantaneously upon addition of penicillin G and then declines gradually in a similar time interval (~500 s).
Most deletion mutants demonstrate the same sensitivity to CG-1521 as in the initial screen, however, the sensitivity of the hfi1Δ mutant is enhanced compared to the screen and yeast deletion mutants ngg1Δ and spt3Δ show slightly increased sensitivity.
While the individual point mutants have rates of cleavage slower than (A89C) or comparable to (I94C) that of the wild type, the A89C/I94C double mutant is enhanced, in line with the A92P cleavage rate.
Bin-3 (bicoid-interacting protein 3) is also implicated in this repression and the levels of embryonic lethality of a bin3 bcd double mutant are enhanced by a pAbp heterozygous hypomorph [ 33], but it is not clear whether dPABP contribution to cad mRNA repression is direct.
