Sentence examples for mutant were engineered from inspiring English sources

The pEGFP-SHP2 WT and C/S mutant were engineered by inserting a coding region into the SalI and BamHI sites of pEGFP vector (Stratagene).

To investigate this possibility, three additional IsdBN1 protein constructs (i.e., IsdBN1 F164D and Y167D single mutants, and F164D/Y167D double mutant) were engineered, with F164 and Y167 replaced by aspartic acid.

Escherichia coli BL21 DE3)/ΔpgiΔzwfΔgalU mutant was engineered by overexpressing thymidine diphosphate (dTDP -d-glucose synthase (tgs), dTDP -d-glucose4,6-dTDP -d-glucose), and a synthaseinotgsnsferase (wecE) from different sources to produce a pool of dTDP-d-glucose,6-dhdeoxy-d-gandctose in the cell cytosol.

This Pfu DNA polymerase mutant was engineered to overcome uracil stalling.

The Ffh(E153C) single-cysteine mutant was engineered using the QuikChange mutagenesis kit (Agilent, Santa Clara, CA).

Each mutant was engineered using the QuickChange in vitro site-directed mutagenesis system (Stratagene) according to the manufacturer's instructions using the primers described earlier.

The sgo1-3A mutant was engineered to disrupt the binding site for PP2A-Rts1 (Xu et al., 2009) whereas sgo1-100 and sgo1-700 were isolated in a screen due to their inability to sense a lack of tension (Indjeian et al., 2005).

To confirm the model, double and quadruple cysteine LexA mutants were engineered.

Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.

VP1 mutants were engineered and characterized for RNA synthetic capacity in vitro.

The low-fidelity mutants were engineered by knocking-in DNA sequences that direct changes of leucine 2618 to either phenylalanine (L2618F) or methionine (L2618M) of Pol ζ.