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Crystal structures of the mutant were determined in two space groups, P21212 and C2, from crystals grown in the same crystallisation set-up.
Differences in e20 binding to wild type H77 and to each mutant were determined by FACS, following the protocol described above.
Each of these residues was mutated to an Ala, and the kinetic parameters for each mutant were determined.
The expression pattern of the GhMYB80 gene in cotton anthers and its capacity to rescue the male sterile atmyb80 mutant were determined.
Incorporation of labeled amino acids and relative protein levels between the WT and cdpk1 mutant were determined in seven and five independent biological replicates, respectively.
The spectra of the intermediates in the O2 reaction of the Tt ba3 G232V mutant were determined on the basis of a proposed kinetic scheme.
Similar(48)
Here, the structure of this P47H mutant was determined in complex with NADH to 2.5 Å resolution.
The sequence of the disrupted ORF for each attenuated mutant was determined by either cloning or PCR-amplifying and sequencing the flanking regions.
The flgE mutant was determined to harbor a single transposon insertion after hybridization with the transposon vector pUTminiTn5Km2 (Table 1) (data not shown).
In vitro growth rate of S. epidermidis 1457 and its isogenic ACME-excision mutant was determined in tryptic soy broth as measured by OD600.
Similarly, the output ratio of the parent and mutant was determined for each rabbit tissues (lung, spleen, kidney, and blood) by transferring 144 CFUs onto TSA containing 300 µg/ml spectinomycin.
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