Sentence examples for mutant were constructed from inspiring English sources

Six recombinant strains expressing wild-type CtXR or an NADH-specific mutant were constructed and evaluated regarding effects of expression mode, promoter strength, biocatalyst concentration and medium composition.

A strain 13 pfoA-null mutant and CN3685 pfoA-null mutant were constructed using our previously described Targetron® technology [6], [8].

Bacterial expression vectors encoding 6His-cdc25CWt and each T-A mutant were constructed in pET101d using the Topo Challenge system (InVitrogen, Les Ulis, France) according to the manufacturers instructions.

Plasmids of FADD and its mutant were constructed as described.

Accordingly, the three permutations of the R141E/Y288A/A306F mutant were constructed.

The CNM-related mutation K436X and the exon10-deleted mutant were constructed by standard primer-directed PCR mutagenesis.

Coa3-null mutant was constructed as described in Mick et al.33.33

S. coelicolor ΔnucS (gene SCO5388, nucSSco) in-frame deletion mutant was constructed by double crossover recombination using the plasmid pIJ6650 (ref. 40).

Finally, a D52N mutant was constructed introducing an N-glycosylation sequence at an area central to the CR2 dimer interface.

A pyruvate decarboxylase (Pdc -deficient mutant was constructed and evolved for raPdc -deficientnsumutant washout ethanol produconstructed

A D95A mutant was constructed as a negative control to substitute for W93A.