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The spore pigmentation and germination defects of the Δsec22 mutant were complemented in the mutant strain carrying the construct that expresses sec22 under its own promoter and terminator regions (strain SEC22-GFP-NA, Figure 3 and Figure 4).
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Finally, a benQ null mutant was complemented with butyrate starter unit biosynthesis genes from the alnumycin biosynthesis gene cluster, which resulted in a great (10×) enhancement in the production of butyrate-primed hexacyclic benastatin derivatives.
The mutant was complemented by transformation with a recombinant vector made of full-length cDNA of the WT AtPIP1 4 gene fused at the 5′-terminus to the gene promoter and fused at the 3′-terminus to the coding sequence of green-fluorescent protein (GFP).
The mutant was complemented with the entire phoPR operon using the replicative plasmid pJUZ1K [16].
The Newman tagO mutant was complemented by introducing a wild-type copy of tagO on plasmid prBtagO.
The hrpL mutant WPP96 was used in this microarray assay, and this mutant was complemented by plasmid phrpL in a previous study [12].
To overcome this difficulty we changed our strategy and the ribH1 mutant was complemented with a plasmid harboring ribH1 under a constitutive promoter before ribH2 mutagenesis (see materials and methods for details).
The dna2Δ pif1Δ mre11-D56N triple mutant is complemented by plasmids expressing Mre11, Dna2 or dna2K1080E, a mutant with defective helicase and functional nuclease, demonstrating that the nuclease of Dna2 compensates for the absence of Mre11 nuclease in IR repair, presumably in 5' to 3' degradation at DSB ends.
Additionally, while the sreA mutant was complemented with the A. terreus sreA gene, the hapX mutant was complemented with the hapX gene from A. nidulans FGSC A4.
In all cases, the auxotrophic mutant was complemented by only one of the candidate genes (Table 1).
The Δ so2426 mutant was complemented by reintroduction of the wild-type so2426 allele on a low-copy-number plasmid.
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