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Two bacterial strains, A. hydrophila AH-3 WT (WT) and A. hydrophila AH-3 ΔwaaE mutant (ΔwaaE mutant) were chosen for further study because the WT is highly virulent whereas the ΔwaaE mutant completely lost its virulence.
Specifically, genes that appeared to show inverse CjFur/CjPerR regulation with no difference in the Δ furΔ perR mutant were chosen for validation.
Two known long-lived conditions, 0.5M sorbitol treatment and a gln3∆ mutant, were chosen as test cases [ 10, 25], along with WT BY4741 and 4 randomly selected deletion mutants from the yeast KO collection.
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The cdc16-116 mutant was chosen for the current study for reasons described herein.
The daf-18 (e1375) mutation is a hypomorphic allele of daf-18, while the rrf-3 mutant was chosen because it shows an enhanced sensitivity to RNAi feeding [17].
This mutant was chosen because it appears to affect a pathway required for immunity to bacterial pathogens in general but not for survival in the absence of potentially pathogenic bacteria.
The T58A c-myc mutant was chosen as a candidate immortalizing agent in considering that the intracellular levels of c-myc protein play a critical role in proliferation and that the stability and accumulation of c-myc are regulated by multiple Ras effector pathways.
The virK mutant was chosen for further analysis.
The npq1lut2 mutant was chosen because of its high sensitivity to photooxidative stress [ 47, 49].
This mutant was chosen because of its functional association with the initiation factor eIF3h, for which a mutant translatome is already available [ 20].
In line with previous approaches [ 42] the growth cutoff for classifying a mutant as a "Growth" or a "No-Growth" mutant was chosen to be 1/3rd of average growth across the dataset.
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