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Among the 100 top-ranked mutants (R>0.629), 12 mutants, including a rnr4 mutant, were categorized as belonging to a "DNA replication" in the gene ontology (GO) database (GOID: 6260).
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The fat-6 mutant was categorized by a particularly high concentration of alanine.
The p.T599I mutant was categorized as an intermediate-activity mutant by direct measurement of MEK phosphorylation using ATP at a physiological concentration, but showed a slightly deceased basal kinase activity (0.84 fold) by measuring ERK phosphorylation using BRAF kinase cascade assay with ATP at a sub-physiological concentration.
The 9 mutants were categorized into four groups according to their drug sensitivity.
The 12 mutants were categorized into four types.
The differentially expressed genes that were similarly affected in both mutants were categorized into functional groups based on Gene Ontology.
A large proportion of let-60 lf) let-60 lfere categorized with CL but exhibited mutantsphenotypes (see Additional file 1: Figure S1) compared to neuron-specific let-60 RNAi strains described belowererobably due to n2021 being a weak allele.
In the previous study by Bennett et al., diploid mutants were categorized as gamma sensitive if there was (a) reduced survival in pronging assays or (b) a slow recovery from gamma-induced damage (producing small colonies) even if the number of surviving colonies was similar to WT cells [ 27, 28].
When the parts of mutants are categorized by degree of constraint, we find that weakly constrained parts are significantly more complex than more constrained parts.
A total of 25 mutant lines were categorized in this class and we further distinguished two subgroups, a first group of 19 alleles strongly interacting genetically and a second group of six single alleles that will not be described further.
In comparison, the KIT-mutant GISTs were separated into two groups, one of which was shared with IGF1Rlow wild-type GISTs (Fig. 3b), while PDGFRA-mutant GISTs were categorized in a single group characterized by high relative expression of PDGFRA, IGF1, and IRS1.
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