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A reduced theoretical pKa value and a lowered experimental pH optimum of hydrolytic rates for the D346A mutant were associated with a 9-fold increase in VmS/VmH.
Among missense mutations, those at codon 179 and the R248W mutant were associated with the highest mortality rates.
We found only 21.3% (217/1 018) of differentially expressed genes in hlp1-1 mutant were associated with HLP1 binding, suggesting that a large portion of altered gene expression is indirectly caused by HLP1 mutation (Supplementary information, Figure S11).
We also compared the two genotypes under irrigated and rain-fed field conditions over three years, finding that the greater oxidative stress and corresponding molecular changes in the pale-green mutant were associated to a yield reduction.
We found that although the WT and the ΔWR TWIST1 mutant were associated with the IL8 promoter, the S144R/K145E DNA binding mutant is not presented at the same region, confirming that these point mutations indeed disrupt the DNA binding ability of the protein.
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In this respect, it can be speculated that the unusual leaf variegation phenotype in the z3 mutant is associated with metal ions which play a key role in chlorophyll biosynthesis.
Elimination of activity toward primed substrates only using the GSK-3 R96A mutant was associated with a defect in axon polarity (axon branching) compared to an overall reduction in axon growth induced by a kinase-dead mutant.
The delayed activation of the SA defense pathway in the mutant was associated with its susceptibility.
Overexpression of a normoxically stable HIF-1α mutant was associated with mildly increased luciferase activity.
This result, however, is in contrast to the recent report that S935A mutant was associated with punta localization in COS-7 cells [24], [25].
More recently, Men et al. [27] showed that defective sterol profiles in the cpi1-1 mutare associatedated with defective PIN2 endocytosis and polar localization following cytokinesis.
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