Sentence examples for mutant were amplified from inspiring English sources

The DNA fragments encoding the mature region of SaAraf43A and the catalytic domain of SaAraf43A (the CBM42-deleted mutant) were amplified by PCR and then cloned into the expression vector pET30, respectively.

The mouse miR-9-2 gene promoter sequence and its E-box motif mutant were amplified by PCR and cloned into the pGL3 fly-luciferase construct (Promega, Madison, WI), respectively.

To validate the candidate gene models, full-length genomic DNA of each candidate gene in Hwacheong and the sug-h mutant was amplified by PCR.

The BUD3-M16-ΔDH mutant was amplified by PCR from BUD3-FLΔDH.

To construct a YFP-tagged Proteasome Inhibitor Reporter (PIR) protein, cDNA encoding a full-length human p53 R175H mutant was amplified by PCR from a cDNA clone, and three consecutive lysine residues in the bipartite Nuclear Localization Signal NLSS) were replaced with alanines by PCR-based, site-directed mutagenesis [25] [26].

The coding sequences of wild-type mouse eIF2α, phosphomimetic (S51D) mutant was amplified by PCR from a mammalian expression vector (kind gift of David Ron).

A construct encoding human AMPK-α2 (D157A mutant) was amplified using primers designed to encode a 5′-Kpn1 site and a C-terminal FLAG tag followed by a 3′-Xho1 site.

The plasmid containing the A25T TTR mutant was amplified in E. coli DH α5 cells and further purified using the Wizard Plus SV Miniprep DNA Purification System kit (Promega, Fitchburg, WI, USA).

Briefly, STING mutants were amplified by PCR or overlap PCR from HA-STING construct, and inserted into pCMV-HA vector with a HA tag at the N-terminus of each STING mutant.

The full-length coding sequence and eIF3f mutants were amplified by PCR to introduce BamH1 and EcoR1 sites on each side of the open reading frame and cloned as BamH1 and EcoR1 fragments into pGEX-3X.

CYLD truncation mutants were amplified by PCR and cloned into CMV promoter-driven pCS2 construct.