Sentence examples for mutant we introduced from inspiring English sources

To find such a mutant we introduced, using recombineering [30], a set of five alanine substitutions into the essential region of CIII within the λ genome.

In order to quantify the surface expression of the G88R mutant, we introduced an extracellular HA epitope in the P2-M4 linker of TASK-4.

To ask whether this elevated Wg expression contributes to the defects in the mutant, we introduced wg mutant alleles into the miR-965 (KO1/KO2) mutant background.

In order to elucidate the structural entity responsible for the attenuation of the mptC mutant, we introduced the M. smegmatis mptC (Kaur et al., 2006) in the mutant, which restored mannan core branching whereas elongation of monomannoside caps was still defective (Fig. S1).

To make plasmids encoding the mad3-ken mutants we introduced point mutations into pKH535 using the Stratagene QuikChange method.

To confirm that a mutation in LOV1 caused the flowering time change in lov1-4 mutants, we introduced the LOV1 gene into lov1-4 mutants by crossing pLOV1 LOV1 HA and lov1-4 plands and then determined whether the early flowering defect was rescued.

Into these mutants we introduced GSK3 mutations, and in neither case armXM19, GSK3 (Fig. 2 C-C"'), nor armF1a, GSK3 (Fig. 2 E-E"') did the additional mutation have an effect on epithelial patterning.

To test whether the different Arabidopsis CRTs could restore the growth defects in the Atcrt1b single, and Atcrt1a crt1b double mutants we introduced two constructs with either an AtCRT1a cDNA or an AtCRT3 cDNA driven by a constitutive 35S promoter into the two mutant lines.

In order to distinguish individual transposon mutants, we introduced 21 bp short oligonucleotide tags that have been used in a previous study.

To display an altered anti-inflammatory capacity of the IRAK-M DD mutants we introduced them in THP-1 macrophages and H292 lung epithelial cells.

To further understand the molecular basis of increased glial phagocytosis in puc mutants, we introduced a reporter for dJNK activity, TRE-eGFP that contains Drosophila AP-1-binding sites fused to the eGFP gene.