Sentence examples for mutant we integrated from inspiring English sources
In order for ECF to simulate the flux distribution in the central metabolic pathways of the pykF knockout mutant, we integrated the enzyme activity data (Table 2) into the EMC vectors for wild type according to the power law formalism of Eqs.
To determine whether translation of Tfc6p was affected in tfc6 promoter mutants, we integrated nine copies of the myc epitope tag coding sequence onto the 3′-end of the TFC6 gene in wild type and tfc6 mutants to create carboxy-terminal 9 × -myc epitope tagged strains.
We integrated this mutant into yeast under the control of the Gal1 promoter and repeated the experiment shown in Figure 4.
We integrated the mutants at the endogenous locus without the GFP tag and under the natural promoter.
Therefore, we integrated a pTET-UME6 construct into the ADH1 locus of the eed1Δ mutant and analyzed the phenotype of eed1Δ after forced expression of UME6.
We integrated the cement plant 10 days ago.
We recovered mutant clones correctly integrated within the follicular epithelium and these were of the same size and frequency as twin spot control clones (see Sce1 mutant clones as an example in Figure 1I I″, encircled and data not shown).
Each mutant was integrated into the yeast genome at the URA3 locus in a RIF2 / rif2 Δ heterozygous diploid and verified by sequencing.
Constitutive Ca2+ entry through the mutant channels, integrated over many hours, is therefore sufficient to stimulate NFAT and c- fos gene expression, at least in a sizeable fraction of the cells.
We reasoned that if each mutant integrated one new vector, the pre-existing barcode should account for exactly half of all barcode counts after transfection and drug selection.
