Sentence examples for mutant we identified from inspiring English sources
In the J4 mutant we identified a T66G substitution that results in an I22 M mutation (additional file 9).
Taking advantage of the temperature sensitive phenotype of the eso1-G799D mutant, we identified several gene deletions that allowed rescue at nonpermissive temperature.
By further characterizing this previously identified mutant we identified a gene that reduces performance of M. persicae and also provided clues about the mechanism involved.
Using genetic screening for second-site suppressors of plastid protein import1 (ppi1; an Arabidopsis atToc33 mutant), we identified SUPPRESSOR OF PPI1 LOCUS1 (SP1), a RING-type E3 ligase protein.
Two of the human HSF1 mutants we identified in LZ1-3 (M161I, L189P) as well as the mutant we identified in LZ4 (L402P) occur at key hydrophobic residues, and are conserved across a variety of species from yeast to humans.
We next addressed if rye interacts with other known sleep mutants, in particular with a mutant we identified previously, sleepless (sss), that has an extreme short-sleeping phenotype (Koh et al., 2008).
From a library consisting of 2126 knock-out mutants, we identified 3 mutants with high-flux and 95 mutants with low-flux phenotypes that did not have severe growth defects.
In fact, none of the hypercytotoxic transposon mutants we identified through our screen were in genes required for O-antigen synthesis.
The four mutants we identified as being more cytotoxic to macrophages and phagocytosed via an actin-independent pathway are all normally involved in synthesis of the LPS core.
However, as we discuss in more detail below, there is indirect evidence in other Gram-negative bacteria that mutations that alter the LPS may have phenotypes similar to the four mutants we identified in this manuscript.
Moreover, by employing a variety of protein mutants, we identified the Ig-like domains 4 or 5 of MAG as necessary and sufficient for growth arrest, whereas abolishing MAG's ability to bind to sialic acid did not interfere with its inhibitory activity.
