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This is a biologically inactive mutant we found incorporating into exosomes at high levels also when fused at its C-terminus with foreign proteins.
Following sequence alignments of the wild type with the mutant, we found a C to G change in the At3g21200 gene of pgr7 (Figure 6B).
In the dnaA46 mutant, we found increased expression of the dnaA operon at the high temperature (data not shown) confirming autoregulation of this gene [31].
Using our DgRNAi mutant we found that robo and Sema-2a enhanced the Dg phenotype and Sema-1a and lea (robo2) suppressed the Dg phenotype (Table 1; Figure 4 and Figure S4).
In the Neurod1 CKO mutant, we found single rows of Fgf8 positive IHCs only in the basal half of the cochlea (Fig. 8E,G) whereas in the apical half, multiple rows of Fgf8 positive IHCs was observed as well as ectopic expression in some IHCs replacing OHCs (Fig. 8E,F,H,I) consistent with the observation of Myo VIIa expression (Fig. 5F,F').
Despite the uncertainty about the molecular nature of this mutant, we found that no detectable PIP3 was produced in age-1(hx546) larvae after H2O2 stimulation.
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When applying the same analysis to several barnase mutants, we found that single mutations may drastically change the free-energy landscape and may significantly alter the population of the two minima.
When we tested the cleavage resistance of V5-MKK7cr_45 V5-MKK7cr_77r_77 mutants, we found both of the mutants were resistant to the cleavage in CHO K1 cells (Figure 2F).
By examining the morphology of motor neurons in the mutants, we found that some VD neuron axons showed defective morphology in several mutants (Figure 1C and Table 2).
When we measured constitutive activity in all the EL1 mutants, we found that E100A, D122A, and S127L, had higher constitutive activity than the wild-type MC4R (Table 1).
In all mutants, we found that asexual development, spore formation and spore germination were indistinguishable from those of wild-type strains.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com