To investigate the effect of a mucA mutant, we focus on the flux reorganisation of the primary metabolism.
To test the effect of dicamba on root growth of AFB mutants, we focused on AFB1, AFB2, AFB3, and AFB5.
Because the double mutant SA179/208 was as effective as, if not more potent than, the single mutants we focused our efforts on the double mutant for the remaining investigation.
Because emp65 Δ and slp1 Δ were SS or SL with all three mps3 SUN domain mutants, we focused on further characterizing the function of Emp65 and Slp1.
Thus, to evaluate potential splicing regulation in mutants, we focused on the ratios of alternative isoforms rather than on levels of individual transcripts.
To identify such mutants, we focused on conserved residues exposed at the surface of CENP-M (indicated with an asterisk in Figure 5A and displayed in Figure 5B; see also the alignment in Figure 3 figure supplement 1, panel E), reasoning that at least a subset of such conserved surface-exposed residues might be involved in CENP-I binding.
In conclusion, by deciphering the ERAD pathway responsible for the processing of V247M mutant we have focused the interest on the two key elements of the pathway, HRD1 and RFP2.
Having a general entry into Ub fusions and chemical mutants in hand we focused on generating C-terminal Ub fusions, since the combined set of chemical synthesis, ligation, and C-terminal modification provides entry into virtually any desired Ub derivative.
To test whether it is possible to detect mutants with circadian abnormalities, we focused on studying nematode mutants of homologs of other organisms' clock genes.
Of the more than 500 temperature sensitive mutants isolated in early screens, we focused on mutants that were characterized as embryonic lethal or reported to have eggshell defects (osmotic sensitivity, irregular shape, and unshelled or thinly shelled embryos).
