Since the peripheral blood counts were unaffected by the double mutant, we focused on the BM compartment and determined that the absolute number of LT-HSC were normal in all single and double mutant mice (Fig. S1B).
To test the effect of dicamba on root growth of AFB mutants, we focused on AFB1, AFB2, AFB3, and AFB5.
Because the double mutant SA179/208 was as effective as, if not more potent than, the single mutants we focused our efforts on the double mutant for the remaining investigation.
Because emp65 Δ and slp1 Δ were SS or SL with all three mps3 SUN domain mutants, we focused on further characterizing the function of Emp65 and Slp1.
Thus, to evaluate potential splicing regulation in mutants, we focused on the ratios of alternative isoforms rather than on levels of individual transcripts.
To identify such mutants, we focused on conserved residues exposed at the surface of CENP-M (indicated with an asterisk in Figure 5A and displayed in Figure 5B; see also the alignment in Figure 3 figure supplement 1, panel E), reasoning that at least a subset of such conserved surface-exposed residues might be involved in CENP-I binding.
In conclusion, by deciphering the ERAD pathway responsible for the processing of V247M mutant we have focused the interest on the two key elements of the pathway, HRD1 and RFP2.
As SP did not produce a change in the pattern of lumbar activation but did affect deletions and pattern formation, especially in Atoh1 mutant, we did not focus on analyzing the frequency effect.
After elimination of number of mutants, we finally focused on three unique suppressor mutants (P1, P4, P5) that prevented YopJ inhibition of the HOG MAPK signaling pathway as indicated by growth in the presence of sorbitol (Figure 2A).
For quantitative comparative analysis of Vps15 and Atg7 mutants we mainly focused on TA muscles that are predominantly composed by fast twitch fibres and thus relatively homogenous in terms of fibre typing.
