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Upon the reintroduction of Duf ΔCT4-flag and Duf ΔCT5-flag into the duf, rst mutant, we find that the average DA1 nuclear number is similar to that shown by the expression of Duf ΔCT3-flag (Fig. 4G, Tables 1 and S1).
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This is a biologically inactive mutant we found incorporating into exosomes at high levels also when fused at its C-terminus with foreign proteins.
In comparative sequencing analysis of PG (CA10g18920) from wild-type pepper and the Soft flesh56 mutant, we found that a point mutation in the 3′ splice acceptor site at intron VIII generated a premature stop codon in the PG gene from wild-type pepper.
Following sequence alignments of the wild type with the mutant, we found a C to G change in the At3g21200 gene of pgr7 (Figure 6B).
In the dnaA46 mutant, we found increased expression of the dnaA operon at the high temperature (data not shown) confirming autoregulation of this gene [31].
Using our DgRNAi mutant we found that robo and Sema-2a enhanced the Dg phenotype and Sema-1a and lea (robo2) suppressed the Dg phenotype (Table 1; Figure 4 and Figure S4).
Employing a substrate-binding-deficient mutant, we found that dimerization was not impaired, confirming that dimerization does not occur through the flexible linker.
Despite the uncertainty about the molecular nature of this mutant, we found that no detectable PIP3 was produced in age-1(hx546) larvae after H2O2 stimulation.
Analogous to the previously characterized S399F mutant, we found that the missense mutation I403T led to retention in the ER and loss of catalytic activity.
Although we also observed that WRN expression failed to correct the HU sensitivity of the sgs1 mutant, we found that WRN expression can restore the top3 slow growth phenotype in the sgs1 top3 background.
For Δ slr0947 mutant, we found that it contained trace amount of original wild-type band in the DNA gels even after more than ten passages, it may worth further investigation whether slr0947 is a lethal gene for the condition.
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