Sentence examples for mutant we estimate from inspiring English sources

For each mutant, we estimate its initial population size c i and the corresponding growth rate r i.

Nevertheless, it should be noted that the HPLC profile of the wild-type enzyme was obtained with one-tenth the protein concentration of that of the A31Y mutant (we estimated that the A31Y mutant was approximately 2.5-fold less active).

Since many of the cell lines displayed no mutants, we estimated the rate using a single hypothetical mutant.

We use these mutant libraries to generate pools of mutant influenza viruses, which we estimate incorporate at least 85% of the possible HA codon mutations and 97% of the possible amino-acid mutations.

Fitting the data to allow for a two-step process involving UBA dimer dissociation and folding by ubiquitin-binding we estimated that the mutant binds at least 6-fold weaker than the wild-type UBA (wild-type UBA Kd = 19 μM and I424S-UBA Kd = 119 μM).

We estimated that purR mutants are present in the unselected cultures at a mean frequency of 0.35 mutants per culture or ∼2.7 × 10−5/10 cells.

First we estimated the levels of Kelch expression in mutants for cul3.

Based on the enzyme kinetics of the wildtype protein kcat/Km of 332/M/s and that of the mutant protein at roughly 3.5/M/s we can estimate that the mutant protein has a roughly 100-fold decrease in enzymatic activity.

Because we have a set of known regulators from KEGG, we can compare the mutant phenotypes to estimate how likely it is that an unknown gene belongs to the same pathway.

A recent method called FATHMM [ 39] also uses an MSA to identify conserved amino acid residues, but builds an HMM from the MSA and computes the differences in model probabilities between the wild-type and mutant residues to estimate the impact of the substitution.

We retained for this study the single Ara+ mutant whose estimated fitness relative to its source sample was closest to unity (and, in all cases, not significantly different from 1).