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In our α-catenin-ΔVBS mutant, we do not see any Vinculin present in any of the junction types.
As we do not yet have a stable tardbpl mutant, we do not yet know what specific role tardbpl-FL may play in zebrafish development.
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However when we triggered the cells with the ΔphoP mutant, we did not observe ROS production or pH changes.
It is possible that the previously published data represent extreme phenotypes of the ΔmamK mutant we didn't meet in our laboratory's conditions.
The LAMP1 protein associates with late endosomes/lysosomes and when detected in conjunction with MEKK1 or the PHD mutant, we did not observe a difference in co-localization.
Consistent with the viability of the B-box∆ epe1∆ double mutant, we did not observe silencing of rrb1+/ SPBC1711.07.
Since we could not reach a clear conclusion on the seminal retrograde transport mutant, we did not try the experiment on the IFT27 RNAi strain.
As SP did not produce a change in the pattern of lumbar activation but did affect deletions and pattern formation, especially in Atoh1 mutant, we did not focus on analyzing the frequency effect.
Despite the reduced responsiveness of a wide range of defence-linked genes in the aos mutant, we did not observe any improvement in aphid fitness in comparison to wt plants.
Classes of mutants we do not expect to isolate are those losing cis-acting sites (such as the origin of replication) and dominant negative mutants.
Although we found recombinant MtrA-His6 is more stable in proteasome-defective mutants, we do not know if endogenous MtrA accumulates in mpa or pafA mutants, or if pupylation affects MtrA activity.
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CEO of Professional Science Editing for Scientists @ prosciediting.com