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We chose to focus further genetic characterization on the only miRNA mutant we analyzed that showed a severe lifespan phenotype (mir-71).
To test this idea for the ado1∆ mutant, we analyzed the effect of this mutation on ADE4 expression and found it to be reduced at day 8, consistent with such a model.
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The observation that∼50% of the conditional mutants we analyzed are potentially fast-acting provides additional incentive to isolate more TS alleles.
Background/Aims: Hepatitis B virus mutants of the polymerase gene are frequently selected during lamivudine therapy for chronic hepatitis B. To study the biology of these mutants, we analyzed their replication capacity in the duck hepatitis B virus (DHBV) infection.
Most of the dumpy mutants we analyzed were generated by EMS following Jenkins [13] in stocks isoallelic for dumpy.
To confirm known morphology defects of mutants, we analyzed the fungal morphology in the CAM by histology 24 h, 48 h, and 72 h p.i.
To determine the effect on Muc16/MUC16 expression in homozygous mutants, we analyzed the expression of Muc16 mRNA and protein by RT-PCR and immunofluorescence, respectively.
To assess the retention process of the N-glycosylation mutants, we analyzed their co-localization with the ER-Golgi intermediate compartment (ERGIC) marker, ERGIC-53.
The number of IMAs increased by 138%, which is the highest value amongst the mutants we analyzed (Figure 3A and 3B).
To find out whether Pio was indeed reduced in γCOP mutants, we analyzed its expression using an anti-Pio antiserum (Figure 5; [8]).
To examine whether the spatio-temporal expression pattern of sec24d correlates with the developmental defects in bulldog mutants, we analyzed RNA samples from consecutive stages by RT-PCR and embryos by in situ hybridization.
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