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No hypersensitivity was observed when the cydKO mutant was tested against 15 17.
Further, each mutant was tested for its binding energetics with respect to the two substrates, dephosphocoenzyme A and ATP.
The tir1-1 afb5afb5 plants were crossed and the resulting homozygous double mutant was tested for dicamba resistance.
Each Nse3 mutant was tested for its ability to interact with both Nse1 and Nse4 using the yeast two-hybrid system.
Each mutant was tested in a functional assay that measured FSHβ-luciferase activity in a stably transfected pituitary gonadotrope cell line (−338 FSHβ-Luc) [59].
In the current study, the TEM8 L56A mutant was tested for its ability to block intoxication and was found to be very similar to CMG2.
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Multiple mutant versions of Cas9, including Cas9D10A, Cas9H840A, and a Cas9D10A,H840A double mutant, were tested.
Two independent isolates of the deletion mutant were tested to ensure that the phenotypes observed were not due to off-target effects.
By contrast, there was an increase in fluorescence when an equimolar mixture of both mutants was tested, indicating that CaM bridges the two proteins41 (see supplemental Fig. 6D).
Lack of motility of flgK mutants was tested on motility agar.
The expression of a subset of these promoters was tested in planta using recombinase-based in vivo expression technology (RIVET) and fitness of the corresponding mutants was tested.
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