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Each mutant was subsequently analyzed for its thermal, SDS and proteinase K stability in comparison to native BSX.
Each PKA mutant was subsequently tested using standard "plasmid shuffle" assays to determine whether essential PKA function was present in the absence of TPK1 expression [15].
In order to determine how suppression could be achieved from a key catalytic residue outside of the conserved kinase core, suppression of the prkaca-F327A mutant was subsequently examined.
An agp6 agp11 double null mutant was subsequently obtained.
CG2709 was renamed vilya, and the mei-826 mutant was subsequently renamed vilya.
The third mutant was subsequently shown to be an allele of CG8316 as well by Sanger sequencing.
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The loss of distal microtubules and retrograde dying back of motor neuron axons in these mutants was subsequently shown to involve loss of TBCE function at the golgi apparatus where it regulates routing of axonal tubulin [49].
This library, contained approximately 600 individual mutants, was subsequently screened for mutants with beneficial phenotypes such as filamentous and auto-flocculating growth, higher lipid content and phenotypic stability.
The resulting recombinant mutants were subsequently expressed, purified, and crystallized.
While Logan and Victor join forces to fight Deadpool, Kayla is wounded leading the Island's captive mutants to safety; the mutants are subsequently rescued by Professor Charles Xavier.
MeCP2 mutants were subsequently generated by site directed mutagenesis using mismatch primers according to manufacturer's protocol (Stratagene).
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