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Following selection with puromycin, a Δ TbGT3:: PAC mutant was selected and transformed with an ectopic, tetracycline-inducible, copy of TbGT3, introduced into the ribosomal DNA locus under phleomycin selection (Wirtz et al. 1999).
A mutant was selected in the presence of GCV.
An rpoS mutant was selected to determine the effect of the stationary phase master regulator.
Among these five variants, the SLS65 (L202) mutant was selected for further analyses.
The katE mutant was selected on SB-agar plates containing 40 µg ml−1 Km.
The fact that the trkH mutant was selected on the basis of impaired growth on solid CDM (which contains ca. 18 mM potassium) further supports this notion.
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The extra exposed residues in the mutant were selected by an increase of SAP for residues with positive SAP values.
Two positive clones of each mutant were selected to rule out clonal variations.
Cells expressing the constitutively activated mutant forms of KIT mutant were selected according to their ability to proliferate in the absence of IL-3.
Thus, this mutant is selected by host immune pressures.
In each generation, the three kinds of genotypes, WT, heterozygote and homozygous mutant, were selected based on locus-specific PCR amplifications.
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