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In cultures of naïve monocyte-derived macrophages (MDMs), WT PIV5 established a highly productive infection, whereas the P/V-CPI mutant was restricted for replication in MDMs by IFN-beta.
The growth of C. gattii cap59Δ mutant was restricted, and cells appeared in clumps (Fig. 4A, B).
The hyphal growth in the mutant was restricted, almost no haustoria were formed and cell death occurred at the infection sites (Figure 2e,f).
These results raised the question whether this high level of homozygosity in Ugni Blanc mutant was restricted to the top of chromosome 18.
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Since this transcription factor controls the expression of the high-affinity transporter PaCTR3, copper uptake in the mutant is restricted to a low affinity uptake system and results in cellular copper depletion [10], [18], [19].
The models demonstrate that the extent of posterior extension in the Kr mutant is restricted because of decreasing activator concentrations.
Expression of Vas in the vas mutant is restricted to the germarium stage, and this mutant produces wild-type numbers of eggs, which however do not support germ cell specification or posterior patterning (Hay et al., 1990; Lasko and Ashburner, 1990; Schüpbach and Wieschaus, 1986a).
In addition, the observation described in a previous study, that the biomass formation in the starchless pgm mutant is restricted by high respiratory losses in the root [ 48], allows us to hypothesise that the differences we observed in the fresh weight of Cvi and Rsch might also be due to a differential regulation of sink-source interaction both before and after the cold acclimation period.
DOI: http://dx.doi.org/10.7554/eLife.06963.013 To further understand why the dendrite loss in the ire-1 mutants was restricted to the distal dendrites, we investigated where the synthesis and folding of membrane proteins took place in PVD.
We were curious to know if the elongation defect in exocyst mutants was restricted to the elongation zone, or if it represented a more generalized defect (for example, a defect altering the basic structure and overall expansibility of the cell wall) that also influenced cell elongation in the meristem.
Whether the observed loss of DRN 5-HT neurons in Sim1 mutants is restricted to a distinct neuronal subpopulation within this nucleus is not clear.
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Justyna Jupowicz-Kozak
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