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The wild-type phenotype of this mutant was restored by one recombinant plasmid (pOPG361) selected from a genomic library of X. campestris pv.
Expression of Prn in the knock-out mutant was restored by allelic exchange using pSR2.1.
Hemolysis activity of the original mutant was restored by a plasmid carrying the sae operon.
Synthesis of MGLPs in the mutant was restored upon complementation with wild-type copies of the Rv1208 gene from M. tuberculosis or MSMEG_5084 from M. smegmatis.
While the mutant grew only poorly in CDM (containing 18 mM potassium), normal growth of the trkH mutant was restored by adding potassium at high concentration, establishing the importance of TrkH in potassium acquisition.
The bactericidal effect of TO against S. aureus in coculture with the pqsL mutant was restored by supplemental HQNO.
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After shifting cells to 27°C, the intracellular traffic of this mutant is restored [ 19].
More work would be needed to find out why the growth defect of this mutant is restored by the addition of proline, but not by any other amino acid.
Interestingly, we found that the growth-inhibiting effect of rps10b on auxin-treated max2 buds was much stronger than for other genotypes and treatment combinations, such that bud outgrowth kinetics of the auxin-treated double mutant were restored to wild-type.
However, pEXP7::GFP expression in the rhd6-1/prc1-1 rhd6-1/prc1-1 rhd6-1/prc1-1n a subset of epidouble cells which have either formutantroot hais orestoredt a bulged phenotype consintent with a function for EXP7 during the early subset of hair formation.
Even though the catabolic activity of ESCRT mutants was restored under rich conditions, ESCRT mutants still failed to boost the catabolic activity of their vacuoles under starvation, and subsequently stopped to grow and failed to enter quiescence.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com