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Interestingly, a greater amount of the R28D mutant was present in the supernatant.
After the first photo-bleaching, virtually none of the GFP-fluorescent dots that were normally observed could be visualized when ARF1-GTP locked mutant was present.
By contrast with FLAG-α1b-adrenoceptor-eYFP, the FLAG-α1b-adrenoceptor TMI-TMIV-eYFP mutant was present only as the 75 and 160 kDa forms.
The truncation mutant was present in the cytoplasmic fraction as well, suggesting that a pool of mutant protein is nuclear, but has impaired interaction with chromatin and is easily released from the nucleus.
Similar(56)
These data confirmed that the chromophore group, missing in the knock-out mutant, is present in the complementation.
We have not explored if this mutant is present at wild-type levels in cells, or if it is active for RydC regulation of cfa in vivo.
A lower ratio indicates that a particular labeled transposon mutant is present at lower abundance in a culture relative to the abundance of a genomic DNA sample.
Of the 22 SNPs, all three possible genotypes i.e. homozygous wild-type, heterozygous and homozygous mutant were present for 15 of them.
A typographic error for the YXXΦ binding mutant is present in previous publications (Honing et al., 2005; Edeling et al., 2006; Motley et al., 2006).
As seen in Figure 3C, the p.N478D mtPAP also showed a similar pattern of co-localization, consistent with the mutant being present in MRGs.
The parent Aichi/1968 NP and its N334H mutant were present at comparably high levels, but levels were markedly reduced for variants carrying each of the three individually deleterious mutations.
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