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The third H37Rv strain (the parent for a dosR deletion mutant) was obtained from D. Sherman (Seattle, WA) and passaged twice through mouse lungs.
Approximately five milligram of functional N-terminal FLAG-(Gly)5 tagged hFasLECD N184Q mutant was obtained from one liter culture supernatant.
The rice mutant was obtained from KDML 105 rice embryos bombarded with N+ + N2+ ions at an energy level of 60 keV and ion fluence of 2 × 1016 ions/cm2.
Similarly the E. coli ∆dinJ∆yafQ∆mqsR∆mqsA∆yeaG quintuple mutant was obtained by transduction of the knockout mqsRA allele from ∆mqsR∆mqsA (donor strain) in to the triple mutant ∆dinJ∆yafQ∆yeaG (recipient strain) cured of its kanamycin cassette using pCP20, using the P1 bacteriophage.
The ghd10 mutant was obtained from japonica cultivar Wuyunjing 7 (WYJ7) rice plants subjected to EMS treatment.
Microarray dataset for Dam mutant was obtained from Robbins-Manke et al [3].
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If several amplified fragments are visible and no mutant is obtained, increase the annealing temperature.
Atm+/+ (WT) and Atm−/− mESCs were obtained from oocytes of Atm+/− mice17, and NSG mESCs (Prkdc mutant) were obtained from NSG mice68.
Genomic DNA samples from ES clones stably expressing an RFM mutant were obtained after three weeks of continuous cell culture.
myf5 hu2022 mutant were obtained from the Wellcome Trust Sanger Institute.
Among them, 9 nas mutants and 1 s mutant were obtained.
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CEO of Professional Science Editing for Scientists @ prosciediting.com