A PNAG-negative isogenic mutant was not killed.
Unfortunately, an OsIRE1 disruption mutant was not available for the current study.
However, the M535I class 2 mutant was not as deleterious in this regard, which is consistent with its ability to be hydroxylated by PHD2 in vitro (Fig. 5a).
When wild-type enzyme was deliberately contaminated with 1 μM Cu2+ it became less stable and formed aggregates, whereas the double mutant was not affected.
Using the appropriate mutants, we showed that the discontinuous DNA synthesis observed in the lig mutant was not due to the occurrence of mismatch repair (mutS or mutL), nucleotide excision repair (uvrB5), or base excision repair (ung) (A130).
Indeed growth of seedlings expressing non-SUMOylatable FLS2 mutants was not significantly different from fls2 knockout mutant plants implying that the FLS2K/R non-SUMOylatable mutant was not able to sense or respond to flagellin (Fig. 1c, d).
Moreover, the equivalent bBest2 I201T mutant was not stimulated by 10 mM ATP in transiently transfected HEK293 cells (Supplementary Figure 3c), and displayed no affinity to ATPγS in MST (Supplementary Figure 3d).
Cheng et al. (2007) demonstrated that NAAT1 mutant was not able to produce DMA and take up Fe(III) efficiently.
At 5 μM La, inhibition of root elongation in the mutant was not significantly different from that in WT (Fig. 4).
Given the low copy number at 200 and 2000 ng mL−1 anhydrotetracycline, the MIC of d-cycloserine to the mutant was not determined.
As determined by ImageJ (Abramoff et al., 2004), a small fraction of enzyme (3-12%, depending on the mutant), was not PEGylated (Figure 1, lane 5 for Cpl-1C45S D194C). Cpl-1C45S D194C
