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In the second model the clv1-1 mutant was implemented as a loss-of-signal mutant, i.e. the receptor still binds CLV3 but no signal is propagated.
For simplicity, determining a new born as a lethal mutant was implemented as a chance event with the probability of 1 - κ; thus, genotypes were not explicitly assigned as lethal genotypes.
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The clv1-1 mutant is implemented either as a loss-of-signal mutant (loss-of-signal model, Figure 1B) or by adding an interference mechanism acting on the CRN receptor pathway (interference model, Figure 1C).
In the current model, lethality was implemented as discarding a new born individual which is determined as a lethal mutant.
Vortexing was implemented throughout the protocol to help break clumps, because sonication proved to be too harsh to permit consistent recovery of signal from 5A mutants.
Taken together, the RG mutant leaf biomass has potential to be implemented as a dual use crop, particularly as the EMS mutation was generated in the S. bicolor DELLA sweet sorghum background, noted by the abundant sucrose sequestered in the stem during development [ 58].
Finally, a third procedure is implemented by sampling the mutants of the master sequence at progressively higher Hamming Distances.
Recent advances in recombineering using synthetic oligonucleotides enable the rapid generation of mutants at high efficiency and specificity and can be implemented at the genome scale.
This implies that all 44 mutant alleles/42 unique mutations (see Materials and Methods) could be genotyped accurately and thus be implemented in a clinical assay.
These mutants have low sleep levels, but probably have high sleep drive that cannot be implemented.
But will it be implemented?
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