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This mutant was highly vital and exhibited only a slightly reduced specific growth rate.
Compared to the parental wild-type SEZ, the ΔhasB mutant was highly attenuated in mice and provided 100% protection against lethal challenge when administered as a live vaccine.
The experimental observation that the DltA-minus mutant was highly susceptible to cationic mCRAMP and resistant to IRE suggests that the charge on the bacterial cell wall affects electroporation and shows that this approach may be promising for fundamental studies on electroporation.
The F. novicida mutant was highly susceptible to the cationic antimicrobial peptide polymyxin.
The sodA sodB double mutant was highly attenuated (Table 1).
The S. cerevisiae Δsec22 mutant was highly sensitive to cell wall perturbing agent CFW [38].
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In addition, time course analysis of TFEB rephosphorylation upon refeeding showed that the phosphorylation of the M144A TFEB nuclear export mutant is highly efficient, and even enhanced, at early time points upon refeeding compared to WT TFEB (Supplementary Fig. 4A).
Results demonstrate that the mutant is highly biofilm-defective.
B. melitensis vjbR mutant is highly attenuated in both cellular and mouse models of infection [19].
In CaCo-2 cells, the mutant is highly attenuated and the fold replication difference is ∼15-fold (Figure 1B).
A trehalose-deficient tps1Δ mutant is highly sensitive to H2O2 and prone to undergo phagocytic digestion [31].
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