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Since RNP1.1 mimics the behavior of wild-type Lhp1, this mutant was excluded from further analysis.
The causative mutation in each independent mutant was excluded from the corresponding CCS reads to uncover background base substitution errors.
In D125A, the amount of protein expressed was too low for a reliable quantification, and therefore this mutant was excluded from activity analysis.
The atrad21.2 mutant was excluded from this and other assays because, to the best of our knowledge, there are no viable atrad21.2 homozygous mutant knockout lines available [ 42].
Although confocal microscopy revealed that N-glycosylation was not required to target AC8 to the PM (Fig. 4 B ), fractionation studies showed that, unlike AC8, the AC8 N-glycosylation-deficient mutant was excluded from lipid rafts (Fig. 4 C ).
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In contrast, deleterious mutants are excluded from consortia either by competition or by local population collapse.
NA subtypes that did not have any mutants were excluded from the total number of sequences.
Frame shift mutants were excluded from our analysis for the purpose of clarity.
Some of the ST556 STM mutants were excluded from this study simply due to their extremely poor growth.
In contrast, deleterious mutants are excluded from consortia either by competition or by a phenomenon which we term 'local population collapse'.
The genes that were not shared between all three independent mutants were excluded.
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