Sentence examples for mutant was established from inspiring English sources

Once the stability of the mutant was established, the above steps were repeated with more stringent selection criteria in form of 30/40 % walseth cellulose and higher concentration of glucose/glycerol.

In this functional group (COG M), high up-regulation in the rosR mutant was established for the following loci: Rt780_45 and Rt780_39 encoding polysaccharide biosynthesis proteins, and Rt780_44 and Rt780_43 encoding glycosyl transferases (Fig.  2).

Clones stably expressing either wild type RhoA or the RhoA-F mutant were established.

Importantly, two independent clones expressing each G9a mutant were established in order to control for potential clonal artifacts.

However, initial screening of the mutants was established on the index ratio of the zone of clearance.

For that reason, the effect of peptides on CaM mutants was established before examining the Ca2+ dissociation kinetics of wild-type CaM, to identify the rate that corresponds to each lobe's action.

Protocols for iterative rapid mutation of S. mobaraensis with helium-driven ARTP system and effective tube screening method of the mutants were established.

ZHBTc4 ES cell lines stably expressing Oct-4 truncation mutants were established as described previously [ 26].

Rest GT D047E11) (Rest GT) mutants were established by blastocyst injection of the D047E11 GT clone (GenBank Acc.: DU821609).

Analysis of the simulated CG trajectories reveals a straightforward molecular mechanism by which the multispanning topology of EmrE and its mutants is established.

We therefore expect that the collection of moss mutants being established here will help to identify novel genes previously unknown in plants [ 7, 12] and will allow to rapidly link DNA sequence and functional information.