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The ΔrelA Y. pestis mutant was as virulent as wild type Y. pestis KIM5+ (Fig. 6), while the ΔrelA ΔspoT Y. pestis mutant was dramatically attenuated.

However, sporulation of this mutant was dramatically reduced in the presence of high calcium concentrations, suggesting that certain proteins engaged in sporulation require N-glycan outer chains to be fully functional.

In contrast, the NEECK mutant was dramatically slower, with t1/2 >6,134 s.

We found that whereas loss of either set1 or clr3 resulted in increased levels of siRNAs, the siRNA level in the set1Δ clr3Δ double mutant was dramatically diminished relative to wild-type.

Although the growth of Δ pkaA mutant was dramatically reduced in liquid glucose-containing minimal media (data not shown), the growth rate was comparable to the wild-type strain when grown in liquid complete YG media (24 h, wild type = 0.116 ± 0.010 g/10 conidia; Δ pkaA = 0.167 ± 0.1018 g/10 conidia).

The total level of aliphatic glucosinolates of the double knockout mutant were dramatically lower than either single knockout mutant in the leaves, and below the level of detection for all aliphatic glucosinolates in both leaves and seeds (Figure 8 and Table S14, S15, S16 and S17).

Though rec12Δ mutants also form dyads (Fig. 2A) (Davis and Smith, 2003), the level of dyad formation of the dfp1-35 mutant is dramatically higher than that in the rec12Δ cells.

The projection profile of the Y7.53F mutant is dramatically different from that of the WT with 77.2% of the conformations in the negative direction.

The affinity of SU 1-30 for all of W394 mutants was dramatically reduced, while the other mutants bound similarly to wild type SU, indicating that W394 is a key residue for the epitope recognized by SU 1-30 (Table 4).

By contrast, the abundance of ubiquitinated dMfn in the dMfn immunoprecipitates from PINK1B9 null mutants and park25 null mutants was dramatically decreased relative to wt, despite the fact that more dMfn was immunoprecipitated from PINK1B9 and park25 mutants relative to wt (Figure 3).

In contrast, pulse song of fruC mutants, but not fruA or fruB mutants, was dramatically reduced when the flies were deprived of visual cues.