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Exact(27)
Here, the structure of this P47H mutant was determined in complex with NADH to 2.5 Å resolution.
The sequence of the disrupted ORF for each attenuated mutant was determined by either cloning or PCR-amplifying and sequencing the flanking regions.
The flgE mutant was determined to harbor a single transposon insertion after hybridization with the transposon vector pUTminiTn5Km2 (Table 1) (data not shown).
In vitro growth rate of S. epidermidis 1457 and its isogenic ACME-excision mutant was determined in tryptic soy broth as measured by OD600.
Similarly, the output ratio of the parent and mutant was determined for each rabbit tissues (lung, spleen, kidney, and blood) by transferring 144 CFUs onto TSA containing 300 µg/ml spectinomycin.
For the rabbit co-infection model, the input ratio of the parent to mutant was determined by transferring 144 CFUs of the mixed inoculum onto TSA containing 300 µg/ml of spectinomycin.
Similar(33)
Crystal structures of the mutant were determined in two space groups, P21212 and C2, from crystals grown in the same crystallisation set-up.
Differences in e20 binding to wild type H77 and to each mutant were determined by FACS, following the protocol described above.
Each of these residues was mutated to an Ala, and the kinetic parameters for each mutant were determined.
As illustrated in Figure 4A, the EOT ensemble of each mutant is determined cotranslationally as TMDs exit the translocon.
Statistical significance between wild type and each mutant is determined by two-tailed Student's t -test (*p<0.05; *p<0.051; *p<0.05001).
More suggestions(15)
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