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In this study, a human TNF-α mutant was created and tested for its anti-tumor effects.
For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NH2Tyr) into its active site, replacing the distal histidine (H64) with NH2Tyr.
The Wii version of Crash: Mind over Mutant was created first, with the graphics scaled up for the Xbox 360, and scaled down for the PlayStation 2. A PlayStation 3 version of the game was rumored, but was promptly debunked by Radical Entertainment as a mistake on many press sites' behalf.
CΔ19 deletion mutant was created to remove a predicted nucleic acid binding domain.
An AXXLL containing Vpr mutant was created in the background of the p98CN009.8 Vpr.
To study the localization of PilP in PilQ-containing complexes within the membrane, a pilP deletion mutant was created.
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Once a mutation occurs, a new mutant is created with fitness drawn randomly from the L possible neighbourhood values.
Also, with an increasing population, the probability that a double mutant is created at random is also increased.
Heterozygote mice expressing the I130T mutant were created as described by Fishman and colleagues [ 25] and were bred on a mixed background of CD1 and C57BL/6 (Gja1 tmicei) mice.
A library containing scFv mutants was created by error-prone PCR, displayed on the surface of yeast, and higher affinity clones selected by fluorescence activated cell sorting.
A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in ∼50% of the sequence.
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