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However, we also noted a second phenotype, in which the NES mutant was contained within punctate structures in the cytoplasm, presumably corresponding to autophagosomes or aggregates of LC3.
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The Tn5EZ mutants were derived from S. aureus RN4220, and all 6,725 mutants are contained on individual, 96-well microtiter plates.
In contrast, the dotI mutant was always contained in smooth vacuoles, usually as single bacteria, and without associated RER or ribosomes at all time points (Fig. 4D, E).
The prfA G145S ΔsigB double mutant was confirmed to contain both the prfA G145S mutation and the downstream actA-gus-neo-plcB transcriptional fusion from the prfA G145S mutant [41] by isolating transductants that exhibited neomycin resistance and a blue colony appearance on BHI agar containing 50 µg/ml 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid (x-gluc).
Synthesis of c-di-GMP was observed when the mutant was transformed with plasmids containing y3730 or hmsT, but not with M-y3730 (Fig. S1).
To determine if MoVam7 can substitute for the functions of S. cerevisiae Vam7, the yeast Δvam7 mutant was transformed with pYES2 containing the MoVAM7 gene.
The chloroplasts in the yellow/white sectors of fgl mutant were damaged and contained many plastoglobules and unstacked grana thylakoids (Fig. 2b).
An AXXLL containing Vpr mutant was created in the background of the p98CN009.8 Vpr.
The soh715hsr8-1 mutant was transformed with genomic fragments containing the sequence of the six genes in the deletion.
However, the S191A/S216L double mutant was not, even though it contains 21 additional Ser/Thr/Tyr residues.
To verify this hypothesis, the mutant was transformed with a construct containing the pro41 ORF, previously shown to complement mutant pro41 (Nowrousian et al. 2007).
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