Sentence examples for mutant was constructed from inspiring English sources

Coa3-null mutant was constructed as described in Mick et al.33.33

S. coelicolor ΔnucS (gene SCO5388, nucSSco) in-frame deletion mutant was constructed by double crossover recombination using the plasmid pIJ6650 (ref. 40).

Finally, a D52N mutant was constructed introducing an N-glycosylation sequence at an area central to the CR2 dimer interface.

A pyruvate decarboxylase (Pdc -deficient mutant was constructed and evolved for raPdc -deficientnsumutant washout ethanol produ

A D95A mutant was constructed as a negative control to substitute for W93A.

The RR02 mutant was constructed by Hancock and Perego [27].

The mprF insertionnal mutant was constructed following another procedure.

For lmo2123 also a deletion mutant was constructed.

A bipD mutant was constructed by single crossover insertional mutagenesis.

The bacterial mutant was constructed by double crossover method using the construct as mentioned previously [17].

The C129S mutant was constructed by site directed mutagenesis and confirmed with direct sequencing (LGTC).