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To determine the biologic cost associated with the different resistance mutations in vitro, each fluoroquinolone-resistant mutant was competed against the fluoroquinolone-susceptible parent strain R6 (with an independent streptomycin resistance marker) as described by Johnson et al. (6 ).
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TFE3/probe complex formation was competed with 200 fold excess amounts of unlabeled oligonucleotides containing wildtype M box or mutant M box.
The mutants can be competed with the parent to discern the social impact of that specific gene.
Detection of photo-affinity labeling that is competed by CT9 indicates that the mutant Sec61α/γ complex is properly folded.
The complexes were competed out with the corresponding unlabeled UP1B or UP2 consensus GATA oligonucleotides, but not with mutant versions of these sequences.
This binding could be competed away by a 20- to 50-fold increase of the wild-type, but not the mutant, PPRE oligomer.
This is because competition of the beneficial mutant can only occur at the edge of a colony, and as the colony grows, a smaller proportion of the mutant population will be competing with the ancestral genotype (Habets et al. 2007).
"You are competing.
"Rosetta is competing.
"We're competing".
Men are competing".
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CEO of Professional Science Editing for Scientists @ prosciediting.com