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The stress tolerance of each mutant was compared with that of its wild-type parent, and with the most thermo- and UV-tolerant wild-type Metarhizium we have tested to date, M. anisopliae var.
Each mutant was compared to the wild type and, when indicated, to other variants.
To exclude that the observed resistance is the result of generally enhanced replication, the replication fitness of each mutant was compared to that of WT replicon.
Growth of the mutant was compared to the wild-type and found to be indistinguishable in TSB media under standard conditions (data not shown).
This mutant was compared to the wild type and single-mutant plants on the level of glucosinolates, gene-expression and resistance to herbivory by the generalist Lepidopteran insect Mamestra brassicae.
Although all three mutants studied were on different genetic backgrounds, it is important to note that each mutant was compared against heterozygous and wild-type littermate controls on the same background.
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Relative expression levels of transcripts in the dnaK null mutant were compared with wild-type levels.
In contrast, the UP-3 region is diminished in abundance by slightly >2-fold when the rofA::aad9 ΔmsmR mutant is compared to the rofA::aad9 construct.
In a second experiment, population sizes of the gacA mutant were compared to those of Pf-5 and found to be similar.
Thus, JCL260 and JCL260 Δ poxB mutant were compared in flask cultures.
Similar structures and phagocyte compositions were observed when lesions of similar sizes in wt and mutant were compared (Fig. 8A,B).
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