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The oslap6 mutant was backcrossed with the wild type (9311), and the resulting F1 plant was further selfed to generate the F2 population.
A confirmed homozygous T-DNA insertion mutant was backcrossed to Col-0 three times before allowing self-fertilization.
For these experiments the mutant was backcrossed for 6 generations into the CS control background.
The gns2 npr1 mutant was backcrossed to npr1 three times before further characterization.
Each mutant was backcrossed at least three times before we performed genetic experiments.
The sis7-1/nced3-4/sto1-4 mutwicewandbackcrossed thece and the sis7-2/nced3-5/sto1-5 mutant was backcronced once to wild-type Col before physiological assays were conducted.
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All transgenes and the dfoxo mutant were backcrossed at least six times into the wild-type outbred Dahomey population carrying the w 1118 mutation and cured of Wolbachia infection and frequently outcrossed back into the wild-type population.
The ju89 mutation was backcrossed multiple times.
The acs-20 mutants and acs-22 mutants were backcrossed twice with N2.
Hemizygous mutants were backcrossed eight times with C57BL/6 mice to generate transgenic mice with a C57BL/6 genetic background.
With the exception of Ins3, which happened to be sterile, all Ins mutants were backcrossed with an rps15-1 strand and proved to be recessive.
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