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When this mutant was assayed with the Aba+2 substrate, the hairpin defect was completely rescued (Figure 4H).
To explore the synthetic genetic relationship between BUR2 and phosphorylation of Not4p, the not4S/T5A and bur2Δ double mutant was assayed for drug tolerance levels.
Each mutant was assayed in triplicate and the results represent at least six independent transfections normalized to wild-type Rev. Results were analyzed using two-tailed Student's t-test assuming unequal variance among groups to detect significant differences between mutants and wild-type Rev.
Previously this D556N mutant was assayed from crude extracts of fibroblasts from patients.
Three Ara+ mutants were isolated from each clone, and each mutant was assayed in preliminary competitions against the population sample from which it came.
When the Arf6 K3E mutant was assayed for its ability to load GTP, we found that this protein loads GTP at the same rate as wild-type Arf6 in the absence of liposomes.
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To test this hypothesis, S. mutans UA159 and its gcp gene knockout mutant were assayed for their ability to initiate biofilm formation and adherence.
The functional properties of the hGRα-R469X mutant were assayed in human HEK 293 cells by transient transfection experiments.
Each male was recovered into lysis buffer and males scoring as mutant were assayed for a centrally located SNP on each chromosome (Wicks et al., 2001).
However, when cell-free culture fluids from the V. cholerae vc1059 or vc0301 single mutants or the vc1059, vc0301 double mutant were assayed by mass spectrometry, none of the mutants were defective in CAI-1 production.
(C ) PVRL4 mutants from (A ) were tested for their ability to induce anchorage-independent colony formation in triplicate (error bars ± SD). (D ) Cells with full-length PVRL4 or the cytoplasmic region deletion mutant were assayed for viability under conditions of anchorage deprivation by measuring total ATP content in cells cultured on ultra-low attachment plates for 72 hr.
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