Sentence examples for mutant was achieved from inspiring English sources

One nrdAB mutant was achieved by inserting an extra copy of nrdHIEF elsewhere in the chromosome.

Complementation of the ΔsrtA mutant was achieved by electroporation of the mutant with the previously described pSAcomp1 vector [43].

Upon sequencing a single colony for each mutagenesis reaction, coverage of 80 90% of the targeted Sav mutants was achieved after PCR and transformation in E. coli TOP10.

Complementation of the P.a.-kerV mutants was achieved by replacing the mutated locus with the wild-type locus [46].

Complementation of mutants was achieved in trans by placing a wild-type copy of the gene on plasmid pBKJ401, under control of the native gerP promoter (see Methods).

Complementation of the fatty acid sensitivity of the sasF, arcA, vraE and SAR2632 mutants was achieved by individually cloning each gene into the low copy number shuttle vector pSK5630 [75] and transforming each mutant with the relevant plasmid.

The specific assignment of the backbone amide signals in PpcAM58 mutants was achieved using the assigned signals in the wild-type protein [ 27] as a guide.

Satisfactory separation of the lipid A mutants was achieved based on the extracted ion chromatograms of each lipid A species as shown in Figure S-11 in the Supporting Information.

35 Rescue of the generated mutants was achieved by transfecting 4 μg of the respective plasmids into Vero E6 (African green monkey kidney) cells seeded in 6-well plates at 5 × 10 cells/well using Lipofectamine 2000 (Invitrogen, Grand Island, NY) according to manufacturer's protocol.

A transposon-based knockout mutant library of Z. mobilis 8b was constructed, and furfural-tolerant mutants were achieved after adaptation using different concentrations of furfural (Yang et al. 2014b).

The Dat mutants were achieved using the QuikChange® site-directed mutagenesis kit (Stratagene).