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However, many issues remain, including drug toxicity and the emergence of drug-resistant mutant viruses, even in treated patients.
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Even more important, E1B mutant viruses, such as the present CRAds, exhibited better cytopathic effect on cells in S phase [44].
Scientists isolated mutant viruses with that property more than four decades ago.
We further tested the Rac1 interfering cell lines infected with the wild type and mutant viruses.
But it particularly preyed, as many mutant viruses do, on young adults.
Many of these attenuated mutant viruses have entered clinical trials.
No significant differences were found in the replication of the mutant viruses when compared to WT viruses (Fig. 3a, left panels).
Importantly, virus-induced necroptosis is also induced in human HT-29 cells by ICP6 mutant viruses; however, ZBP1 levels must be elevated for this pathway to be active.
For example, circuit tracing requires use of mutant viruses that are much less virulent than wild type PRV.
However, these agents drive selective pressure for mutant viruses that can develop rapidly and have reduced susceptibility to the drug.
Yet these agents drive selection pressure for mutant viruses that can reduce susceptibility to any given drug.
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