Sentence examples for mutant versions were from inspiring English sources

Mouse Chordin and its VWC domains, zebrafish CV2, its fragments and mutant versions were expressed in SF9 cells and purified as described [12].

Plasmids containing the glr-1 promoter, followed either by an rpm-1 minigene, pmk-3 cDNA, dlk-1 cDNA, snn-1, syntaxin-13 cDNA, or rab-5 cDNA (including mutant versions), were generated using standard techniques.

The HA-tagged Lto1 mutant versions were co-expressed together with Yae1-Myc to investigate their interaction.

Mutant versions were created following a modified protocol of site-directed mutagenesis (Liu and Naismith, 2008) using desalted, partially overlapping primers.

Therefore, these candidates and their Pro mutant versions were expressed and investigated by western blotting to probe their steady-state protein levels.

To monitor expression levels of the mouse Wnt3a proteins, 25 pg of the His-tagged wild-type mouse Wnt3a capped mRNA or its mutant versions were injected into zebrafish embryos at the one-cell stage.

The gene is called Kit and mice with a mutant Kit gene have white patches on their tails and feet.In one experiment, mice with two versions of the gene, a normal and a mutant version, were crossbred.

Assays conducted in vitro and in vivo confirmed that the mutant version was completely inactive and was incapable of undergoing self-cleavage to generate the active form of the enzyme.

An electrophoretic mobility shift assay confirmed that an oligonucleotide containing the binding site is recognized by DBP, but a point mutant version is not (Figure 7B).

This indicates that the mutant version is still subject to regulation by Patched1.

When this mutant version was expressed in skin cells it promoted their survival.