Sentence examples for mutant vectors containing from inspiring English sources

Besides, mutant vectors containing four mutated bases on the predicted binding sites were constructed.

In a NER-deficient UvrA − E. coli mutant, vectors containing the stable, reduced cross-link 23 (Scheme 4, R = CH3 ) failed to yield transformants.

Mutant vectors containing 3′-UTR with miR-491-5p-deleted miR-491-5p-deleted miR-491-5p-deleted miR-491-5p-deletedrs designed with the QuickChange primer design software (Supplementary Figure S9) and the QuickChange XL Site-Directed Mutagenesis Kit (Stratagene, Agilent Technologies, Massy, France) according to the manufacturer's instructions.

To compare the packaging efficiencies between AAV-DJ and its three single point mutants, vectors containing double-strand GFP reporter were packaged by PEI-based triple transfection of 293 T cells.

These mutant PINCH1 vectors contained either one or three leucine to alanine (L/A) point mutations in the NES/NLS motif, respectively (Figure 4B).

To further confirm the regulatory role of −541 HRE in SNAI1 transcription, we generated several reporter vectors containing mutant HRE sites (M1, M2 and MM).

Vectors containing two mutants, A623V and I691F were generated by primers 5'- GAT ACC AAA ATT GTC AAG AGG ATG GCT- 3', 5'- AGC CAT CCT CTT GAC AAT TTT GGT ATC - 3'and 5'- AGG GAT GTG TTC TTC CTA CTC AGC AAG - 3', 5'- CTT GCT GAG TAG GAA GAA CAC ATC CCT - 3' respectively.

For generation of lentiviral vectors containing the SOD1 mutant variants fused to AcGFP, we used the lentiviral backbone pRRL156sin-cPPT-hCMV-wpre, containing a multiple cloning site.

The SH-SY5Y cells were transduced with lentiviral vectors containing WT or mutant HSPB1 as described in " Materials and methods".

The complementation of each of the three mutant strains was accomplished using integrative vectors containing the gene of interest expressed from either its native promoter (sven0913 and sven0915) or from the constitutive ermE* promoter (sven0914).

We constructed luciferase reporter vectors containing wide-type or mutant 3′UTRs of NR2F2 (Fig.6B).