Sentence examples for mutant using the from inspiring English sources

(A) Construction of a single-gene deletion mutant using the λ Red system.

Plasmids with and without inserts were separately transformed into the S. cerevisiae Δtkl1 mutant using the lithium chloride method (Finlayson et al. 1991).

Analysis of total cellular lipids of Golgi myo-inositol transporter TbHMIT mutant using the shotgun technique showed that TbHMIT contributes to bulk PI production but not to GPI-anchor biosynthesis [58].

EC and cell-associated carbohydrate extracts were prepared for the PA14ΔpelC mutant using the same procedures as for the wild-type (WT) strain.

Site-directed mutagenesis using the Stratagene Quick-Change II kit (Stratagene) was used to generate the P58S mutant using the wild type cDNA.

The mixture model describes each mutant using the resulting proportions of each wild-type state (Figure 7C), and genes that could not be accounted for in the model were revealed (Table 4) [36].

To gain insights into the function of ezh1 in zebrafish development, we generated ezh1 loss-of-function mutants using the transcription activator-like effector nuclease (TALEN -based TALEN -based

A novel STM approach was designed to allow for a higher throughput assay of mutants using the Luminex xMAP® system.

A new system was developed for producing conditional-lethal vaccinia virus mutants using the tetracycline controlled gene expression system from bacteria.

The resulting binary and empty pCAMBIA2300 vectors were introduced into the ygl138-mediated transformation method (Liu et al. mutants using the Agrobacterium tumefaciens2007).

Chromosomal DNA was obtained from the selected mutants using the GENOME DNA KIT (MP Biomedicals).