Sentence examples for mutant uses a from inspiring English sources

Further while the Grx mutant uses a single active-site cysteine, its mechanism is distinct from the monothiol mechanism as described above which allows the formation of GrxSS.

We previously constructed an HcDPPIVA-deletion mutant using a Ku-deficient strain of H. capsulatum.

We mapped the 88.21 mutant using a CA panel and identified a mutation in the catalytic domain of the aldh1a2 gene; therefore we named our mutant aldh1a2um22.

Although we created this mutant using a clathrin cryo-electron microscopy model and a yeast temperature-sensitive mutant as a guide, the resulting temperature-sensitive clathrin showed an altered phenotype compared to the corresponding yeast temperature-sensitive clathrin.

The mapping performed on the 78730 mutant used a subset of the available markers on both arrays: 30,000 SFP markers were used, although, permutation testing suggests that 100,000 SFPs could be used with a false discovery rate of 0.05.

We further analyzed the function of the D-CblL-mPR mutant using a constitutive expression promoter, HS83 [40], which was also used in rescue assays by D-CblL and D-CblS.

Next, we sought to selectively elevate myosin activity in a fra mutant using a constitutively active Myosin Light Chain Kinase (U-ctMLCK) that increases myosin activity by phosphorylating the Regulatory Light Chain of myosin II [56].

We also performed transgenic rescue of the bub-1 mutant using a PCR product which encompasses the region from 1.40-kb upstream to 0.82-kb downstream of the bub-1 locus.

To pinpoint the defect in daughter abscission at the molecular level we evaluated the development of the cytoskeleton in the MORN1 mutant using a battery of markers to highlight different aspects of the budding, maturing and mature cytoskeleton (Fig. 5).

As for peg2 no additional alleles were available, we complemented the peg2 mutant using a genomic construct.

The signaling kinetics of these mutants was measured along with wild-type melanopsin and the previously characterized phospho-null melanopsin mutant using a functional calcium fluorescence assay.