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(C) Diagrams of the RNF125 stop mutants used in Fig. 2. (D) The C2HC ZnF mutant used in Fig. 3.

Among them, severe sugary-type endosperm mutants, such as EM914 (Nakamura et al. 1997; Wong et al. 2003), #1 1 (Tanaka et al. 2004), and OsISA1 suppression and OsISA2 over-expression transgenic lines (Utsumi et al. 2011), were similar to the sug-1 mutant used in this study.

The ΔisdBH mutant used in this study has been previously described [8].

DNA sequencing revealed that the ausA deletion mutant used in the original study has an 83 nt-duplication in saeS.

The hypomorphic dcl1 mutant used in this study, dcl1-7, has a mutation in the helicase domain [25].

The β-catenin-ΔGSK-GFP mutant used in photo-activation experiments also revealed that LPA suppresses the gene expression that is elevated by mutating β-catenin (Figure S6).

(F) The RNF125 mutants used in Fig. 7.

The T-DNA insertion mutants used in this study are listed in Supplementary Table 2.

(E) Amino acid sequence of the C2HC ZnF for RNF125 and the mutants used in panels (E I).

We conclude that the RING and C2HC ZnF form a functional unit that is required for binding to the E2. Figure 1: RNF114, RNF114 and the mutants used in this study.

The nomenclature of LinB mutants used in this study is shown in Table 1.