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An increase in the activity of BGL was observed in the double mutant under repressing and de-repressing conditions, showing levels of enzyme activity similar to the ΔpkaA mutant under de-repressing conditions.
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Macrophage killing was significantly attenuated in the conditional mutant grown under repressing conditions compared to the controls.
Thus, under repressing conditions, the mutant produces highly branched hyphae.
Under repressing conditions, the tSEC6 mutant had a striking impairment in filamentation, producing hyphae that lacked the normal branching pattern.
Under repressing conditions, all of the transformants grew robustly.
We found that the expression of this gene is elevated under repressing conditions relative to de-repressing or inducing conditions.
Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.
In contrast, an abnormally elongated septin ring structure was observed in the tSEC6 mutant strain grown under repressing conditions (i.e., with DOX) (Fig. 3C).
To remove these confounding factors, we tested the ability of the GR double mutant K442A R447A to repress constitutively active reporters harboring ~ 150 bp of κBRE-containing promoters.
The T370D mutant significantly repressed ERα and integrin β3 protein levels, while the T370A mutant failed to repress ERα and integrin β3 protein levels.
All mutant proteins repressed the translation of the reporter, although the CIM mutants were impaired to different extents.
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CEO of Professional Science Editing for Scientists @ prosciediting.com