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Recombinant AAV vectors of hybrid serotype 1/2 were produced essentially as described [45], [46] to express either EGFP, human Tau (WT or mutant P301L) or human APP (WT or SLA triple mutant) under control of the human synapsin1 gene promoter [19].
Expression of OsHsfC1a in the hsfc1b mutant under control and salt stress conditions.
The downregulated genes also included those induced in the mutant under control conditions.
We can ask whether high GABA levels that occur in pop2-1 munder under control and even more under NaCl conditions (figure 6A) could not be toxic.
The URDEGs were again subcategorized into up-regulated and down-regulated classes based on their expression in the mutant under control or stress conditions when compared with the WT.
Thus the low evapo-transpiration stress symptom of the esk1-5 munder under control conditions may reflect a different mechanism than that typically induced by the bulk of stress responsive genes.
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(D ) Δ hvyA strains harbouring hvyA catalytic mutants under control of P van on plasmid were tested for sensitivity to φCr30.
Electroporation of Scl −/− ES cells with Scl cDNA constructs (wild-type and mutants) under control of EF1α promoter (pEF1α) and selection of stably transfected ES cells were as described (Schuh et al., 2005 ).
(E ) and (F ) Analysis for the rescue of phototropic hypocotyl bending defects of a pin3 pin4 pin7 mutant carrying wild type and mutant transgenes for the expression of wild type and mutant PIN3 under control of a PIN3 promoter fragment.
Transgenic mice were derived that constitutively express a trans-dominant-negative kinase-defective mutant PKR under control of the beta-actin promoter.
Third instar larvae expressing these mutant proteins under control of the hsp70 promoter were heat shocked and salivary glands were stained with antibodies to Drosophila Lamin C and lamin Dm0.
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